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KMID : 0357319920270050471
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 5 p.471 ~ p.486
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A Serologic Study on the IgM, IgA and IgG Antibody Positive Rates Against Chlamydia trachomatis
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Abstract
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Chlmydial infection in human is a sexually transmittable disease causing urethritis and epididymitis for men and cervicitis, salpingitis and endometritis for women, Ascending infection in reproductive organs may cause sterility and ectopic
pregnancy.
The offspring from infected individuals may also have neonatal conjunctivitis and pneumonia.
As chlamydia is and intracellular parasite, its isolation and identification requires cell culture method which is time-consuming, costly and laborous. Recently, to overcome these drabacks, serological methods such as enzyme immunoassay were
developed
as convenient diagnostic testes.
For the purpose of founding the basis of serological data, the author investigated the IgM, IgA and IgG antibody positive rates against Chlamydia trachomatis by enzyme immunoassay using C. trachomatis antigen, by cell culture methods. IgM and IgG
positive rates against. C.trachomatis antigen were compared with those against chlamydial group antigen purchased from Common Wealth Lab. (Australia). The subjects consisted of infants or children, normal adult females and pregnant women.
The results showed that IgM, IgA and IgG antibody positive rates of hospitalized or noraml infants and children(n=20, average age 2.8yr) were 30%, 25% and 65%, respectively. For normal adult women(n=20, average age 28.4yr), they were 55%, 35% and
70%
and for pregnant women (n=40, average age 28.3yr), 52.5%, 52.5%and 45%, respectively. These results revealed that the IgM antibody positive rates were high in the sexually active female group.
To distinguish the past or reinfection from the recent infection, IgG avidity test was performed. The result of thes test, accompanied by the analysis of the combination of the positive antibody classes(9/13) cases were IgM(-), IgM(-), IgG(+)),
suggested that the high avidity of the children group was attributable to the transferred antibodies across the placenta from infected mothers.
Comparing the C. trachomatis antigen prepared by cell culture methods with the purchased chlamydial group antigen, the EIA showed little difference in IgM and IgG antibody positive rates. This results were regarded to guarantee the usefulness of
the
prepared C. trachomatis antigen for the serologic study of C. trachomatis infection
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